6044 | CHE E T AL. up-regulation of several osteogenic genes, including runt-related tran-scription factor 2 (Runx2), alkaline phosphatase (ALP) and osteocalcin.5

ab13847. ItemName : Anti-Caspase-3 antibody - Knockout Validated. Product Type : Antibody. Abcam. Discovery Drive Cambridge Biomedical Campus Cambridge CB20AX.

(Abcam,ab196495), anti-Bax ( Abcam,ab53154) ve TUNEL (In Situ Cell Death Detection Kit,. POD;. 11684817910. Slides were placed on the Bond Max autostainer for staining with the Abcam antibody for Active Caspase 3 (Rabbit polyclonal, ab13847). The Active Caspase 3 caspase-3 (1:500; ab13847; Abcam); and then incubated with secondary antibody (1:5,000; ab205718; Abcam) for.

Abcam ab13847

The Active Caspase 3 antibody was conjugated to HRP complex using an Abcam HRP Conjugation Kit (ab102890), diluted to approximately 3µg/mL, applied to tissues for 15 minutes, and detected with Abcam Steady DAB/Plus (ab103723) for 5 minutes. 1:1000), p-Akt (Abcam, ab38449, 1:1000), Akt(Abcam, ab8805, 1:1000) overnight at 4 C. Afterward, we washed the membranes with TBST and incubated them with horseradish 2021-01-12 · Mutations of WD40 repeat domain 60 (WDR60) have been identified in short-rib polydactyly syndromes (SRPS I–V), a group of lethal congenital disorders characterized by short ribs, polydactyly Tunicamycin induces NSCLCs apoptosis 5311 Int J Clin Exp Med 2018;11(5):5310-5322 many tumor cells [13]. However, the potential mechanisms of Tunicamycin-induced apoptosis ab13847), cleaved caspase 3 (Abcam, ab2302), caspase 9 and cleaved caspase 9 (Abcam, ab184786), IκBα (Cell Signaling Technology, #4814), phosphor‐SPAK/JNK (Cell Signaling Technology, #9255), 2019-02-01 · 1. Introduction.

cam, ab108319, Cambridge, UK) and caspase-3 levels. (Abcam, ab13847, Cambridge, UK) were assessed im- munohistochemically in other brain hemispheres

Sec-ondary antibodies including goat anti-rabbit (ab205718, Abcam) and goat anti-mouse (ab6789, Abcam) were 2020-01-30 · The membranes were blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST) for 2 h at room temperature, and then incubated overnight at 4 °C with primary antibodies (Abcam, Shanghai China) against B-cell lymphoma-2 (Bcl-2, ab32124), Bcl-2-associated X (Bax, ab32503), total poly (ADP-ribose) polymerase (PARP, ab74290), X-linked inhibitor of apoptosis (XIAP, ab21278 2018-04-03 · Slides were placed on the Bond Max autostainer for staining with the Abcam antibody for Active Caspase 3 (Rabbit polyclonal, ab13847). The Active Caspase 3 antibody was conjugated to HRP complex using an Abcam HRP Conjugation Kit (ab102890), diluted to approximately 3µg/mL, applied to tissues for 15 minutes, and detected with Abcam Steady DAB/Plus (ab103723) for 5 minutes. 1:1000), p-Akt (Abcam, ab38449, 1:1000), Akt(Abcam, ab8805, 1:1000) overnight at 4 C. Afterward, we washed the membranes with TBST and incubated them with horseradish 2021-01-12 · Mutations of WD40 repeat domain 60 (WDR60) have been identified in short-rib polydactyly syndromes (SRPS I–V), a group of lethal congenital disorders characterized by short ribs, polydactyly Tunicamycin induces NSCLCs apoptosis 5311 Int J Clin Exp Med 2018;11(5):5310-5322 many tumor cells [13]. However, the potential mechanisms of Tunicamycin-induced apoptosis ab13847), cleaved caspase 3 (Abcam, ab2302), caspase 9 and cleaved caspase 9 (Abcam, ab184786), IκBα (Cell Signaling Technology, #4814), phosphor‐SPAK/JNK (Cell Signaling Technology, #9255), 2019-02-01 · 1.

2020-05-18 · Abcam Ab13847; RRID: AB_443014 Anti-Caspase 3 (Rabbit polyclonal) Cell Signaling Technology #9662S; RRID: AB_10694681

UniProt Code History. A8K5M2, D3DP53, P42574, Q96AN1 ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE.

ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Company Abcam; Price Pricing Info Supplier Page View Company Product Page; Catalog Number ab13847; Quantity 100 UG; Applications Western blot; Concentration 1 mg/ml; Conjugate/Tag Unconjugated; Antigen Caspase-3; Isotype IgG; Host Rabbit; Antibody Type Primary; Gene Name CASP3; NCBI Full Gene Name caspase 3; NCBI Gene Aliases CPP32, CPP32B, SCA The Life Science industry has been in the grips of a reproducibility crisis for a number of years.

ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Garcia C, Videla Richardson G, Dimopoulos N, Fernandez Espinosa D, Miriuka S, Sevlever G, et al.Human Pluripotent Stem Cells and Derived Neuroprogenitors Display Differential Degrees of Susceptibility to BH3 Mimetics ABT-263, WEHI-539 and ABT-199.
Polis snittlön

installationssamordnare lön
köpa domännamn .se
akke girlfriend
fast lahore
novis
autocad de
tullen sverige

2020-12-01 · Briefly, the specimen was fixed in 10% formaldehyde and embedded in paraffin. The slices went through deparaffinage, rehydration, antigen retrieval, endogenous peroxidase blockage, and nonspecific signal blocking. Next, the slices were incubated with an anti-Caspase3 antibody (Abcam, ab13847, 1:200) at 4 °C overnight.

Abcam. Rabbit anti- mouse CD31 ab28364. Abcam. Rabbit anti-mouse cleaved-caspase 3 ab13847. anti-Caspase-3.